Effects of Industrial Effluent on Zebrafish

Zebrafish embryos are exposed to industrial wastewater in a range of dilutions, demonstrating that they are a very sensitive creature to the toxins found in industrial effluent.

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Industrial waste water is contaminated with dangerous chemicals and compounds, rendering it unfit for human consumption and other uses.Toxic and persistent chemicals have negative consequences on soil, water bodies, agriculture, flora, and animals.As a result, pollution has become a global issue, and ecosystem health is a critical concern.

What are the sources of effluent?

  • Pharmaceuticals
  • Endocrine disruptors
  • Personal care products
  • Surfactants
  • Pesticides
  • Metals

Toxicity Testing in Zebrafish:

Zebrafish is used as a model for toxicity testing due to its similarity with mammals and zebrafish embryos are used mainly because of their transparency which allows for easy visualisation of the toxicity changes.

Zebrafish embryos are exposed to industrial wastewater in a range of dilutions. Several morphological changes are observed such as 

  • Coagulation of embryos
  • Non-detachment of tail
  • lower frequency of the heartbeat
  •  Curved body
  •  pericardial edema and yolk sac edema

The Zebrafish is crossed and the eggs are allowed to fertilize, the embryo is taken and treated with different concentrations of the industrial wastewater placed on 24 well culture plates. E3 medium was used as the test medium.

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It can be concluded that industrial effluents have deleterious effects on zebrafish embryos even at very high dilutions. Exposure of zebrafish embryos to industrial wastewater demonstrates that they are very sensitive creatures to the toxins found in industrial effluent.


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